FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n01_dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12230949
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2078651.6995001777866952TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG188840.1543952149583814TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG323850.050.36375857
TGCCGTC347450.048.4043448
GCCGTCT342550.048.17634249
CCGTCTT346150.047.9784550
AAAAGGG286400.047.89232370
TATGCCG357850.047.5157946
ATGCCGT356900.047.39806447
TGCTTGA347250.047.22189758
CATCTCG343900.046.08062739
GCTTGAA365300.045.6167859
GTATGCC377600.045.16029445
GCATCTC352950.045.01769638
AGGCATC369600.044.4858336
TAGGCAT384150.044.29500235
GGCATCT360450.044.2460637
CGTATGC387800.043.9273544
ACTTAGG411350.043.65489232
CTTAGGC410300.043.63012733
CGTCTTC381000.043.50701551
CTCGTAT378800.043.44607542
Produced by FastQC (version 0.11.2)