FastQCFastQC Report
Tue 25 Feb 2020
H3H2MAFX2_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3H2MAFX2_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138947190
Sequences flagged as poor quality0
Sequence length70
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGATCGACAGCAGTATAGCGACCAGCATTCACATACGATTGACGCATGATATTACTTTCTGCGCACTT3070233922.096408714706644No Hit
TCGAATTCAAGCTTAGATCTGATATCGGTACCCAACAAACCACATTATGTCTTTAGCGATAACTTCGTAT1612871711.607803655475148No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8244470.5933527694946548No Hit
CGGGATCGACAGCAGTATAGCGCCCAGCATTCACATACGATTGACGCATGATATTACTTTCTGCGCACTT1450380.10438354312886787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATC35841750.063.9167371
GGGATCG35952600.063.711292
GATCGAC36055300.063.4111674
TCGACAG36094550.063.37446
CGACAGC36089900.063.2830357
GGATCGA36174450.063.2605933
ATCGACA36249750.063.1600955
AGCGACC35598450.063.15239719
GACAGCA36274400.063.1212928
ACGCATG35669450.063.09890744
GCGACCA35614050.063.08819220
CGACCAG35837750.063.05905521
GCGCACT35513800.063.05685463
ACATACG35954450.063.0000833
TCTGCGC35627100.062.9964760
GACGCAT35722850.062.99421743
GTATAGC36054300.062.99065415
TGCGCAC35651350.062.97471262
ACGATTG35901050.062.96836537
TATAGCG36068200.062.9314116
Produced by FastQC (version 0.11.5)