FastQCFastQC Report
Tue 25 Feb 2020
H3H2MAFX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3H2MAFX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138947190
Sequences flagged as poor quality0
Sequence length10
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTTAGGT6957221150.07097372750036No Hit
AGATCTCGGT44567783.207533739977037Illumina Single End PCR Primer 1 (100% over 10bp)
CTACATGCCT22276271.6032184601934014No Hit
AACGGTCTAT22092511.5899932916959312No Hit
CTGGAACTGT21664671.5592017370052609No Hit
TTGACTAGTA21025541.5132036855153386No Hit
TTGCTGCCGA20901331.504264317975772No Hit
AGTATCAGTT20823281.4986470759142376No Hit
AATATTGCCA20574041.4807093256078083No Hit
CCATCATTAG18967901.3651157680842627No Hit
CCTGACCACT18629891.3407892595740871No Hit
CCAGTCGACG18619981.3400760389612774No Hit
CATTAGTGCG17651201.2703531464004416No Hit
TGAGACTTGC17331961.247377510837031No Hit
CACTATCAAC16910411.217038646121595No Hit
TGTAATCGAC16133541.161127475841721No Hit
TGTCGCTGGT15869431.1421195347671298No Hit
AACCATAGAA15817641.1383922193748575No Hit
TTCAGTTGTC15472501.1135525662663635No Hit
TTCCTACAGC15331921.103435053274557No Hit
AGAGGCAACC15107551.0872871916301439No Hit
CACGGCTAGT14911471.0731753553274448No Hit
ACAGTGTATG14678971.056442379295328No Hit
TCGTGCATTC13547320.9749977671372843No Hit
TCACCAACTT13420750.965888550894768No Hit
CGCGGTGATC13139310.9456333733701272No Hit
ACGGATGGTA12123230.8725063097713599No Hit
GCGGAGCCAA12016580.8648307317334017No Hit
CCTTCGTGAT11870940.854349051607305No Hit
GCCATGTGCG11645120.8380968337682827No Hit
AGTAGAGCCG10670710.7679687512932072No Hit
AGCGCCACAC9398160.6763835958107537No Hit
GGGGGGGGGG7509270.5404405803384725No Hit
CTATAGTCTT6088760.438206774818548No Hit
TGGCCGGATT5101220.3671337290088414No Hit
AGGCTTAGTG2264490.16297486836545597No Hit
AGGCTTAGGG1645740.11844356118320926No Hit
AGGCTTAGTT1569830.11298033447095979No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)