FastQCFastQC Report
Tue 25 Feb 2020
H3H2MAFX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3H2MAFX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138947190
Sequences flagged as poor quality0
Sequence length10
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGCATGAT1592042511.457896341768409No Hit
CTCTCTACAT131713309.479378460262494No Hit
CAGAGAGGAT131328729.451700318660636No Hit
GGGGGGGGGG63762734.588990248741267No Hit
GCTACGCTAT62578584.503767222640487No Hit
TCCTGAGCAT54454653.919089691558354No Hit
CGTACTAGAT48374343.481491061460113No Hit
AGGCAGAAAT34211672.4622066844244928No Hit
TAAGGCGAAT31143572.2413961736109957No Hit
TGGCTAATCA22859371.645184044384057No Hit
TTACCTGGAA22320591.6064081612589647No Hit
CTGTTGGTCC22130641.5927374997651986No Hit
CTCGCTTCGG21709461.562425263871835No Hit
TGGCGCGAAC21441641.5431503148786239No Hit
GAGAATGGTT21269251.5307434428864664No Hit
CGAGGCTGAT20447151.4715770790326888No Hit
ACCGGCCGTA19893661.4317425203057363No Hit
GTCTAATGGC19162471.3791189300049898No Hit
AGAGGCAACC19102851.3748280911618291No Hit
TCTATCCTAA18816951.354251928376529No Hit
TATGTAGTCA18037241.2981363638948007No Hit
AACACTGTTA17723511.2755572818709036No Hit
AGTCAGACGA16861321.2135056491606633No Hit
GACTGAGTAG16765691.2066231782017327No Hit
TGATTATACG16703491.2021466573019577No Hit
ACTCGGCAAT16047711.1549503088187676RNA PCR Primer, Index 48 (100% over 10bp)
CAATCGGCTG15336061.1037330082026129No Hit
CCATCTCGCC15312401.1020302029857532No Hit
CCATCATTAG15151961.0904833699767515No Hit
CCGTATGTTC14906811.0728399761089087No Hit
GGTAACTCGC14809841.0658610656322016No Hit
CAGCCGCGTA14571011.048672520833275No Hit
TCCATTGCCG14203771.0222423353793624No Hit
TGTAATCGAC13616610.9799845538438021No Hit
CTTGCCATTA12273800.883342800959127No Hit
ATTGCGCGGT12196940.8778112029469614No Hit
GATAGGCCGA12131100.8730727120138233No Hit
GTGCAGACAG12071940.8688149792737802No Hit
GAAGCGGCAC11617490.8361083084875628No Hit
GAGTCATAGG10457850.7526492619246203No Hit
CGGTTACGGC6507590.46834988170685565No Hit
CTGCGAGCCA5077390.3654186889277862No Hit
NNNNNNNNNN1920560.1382222987021184No Hit
GGGGGGGGGT1749640.1259212223003574No Hit
GCTAGCTATC1459060.10500824090073357No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)