FastQCFastQC Report
Tue 25 Feb 2020
H3H2MAFX2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3H2MAFX2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138947190
Sequences flagged as poor quality0
Sequence length70
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATATGGACTAAAGGAGGCTTTTGTCGACGGATCCGATATCGGTACCATAATAAGAATAAACTAGATT65479574.712550861949781No Hit
TTAATATGGACTAAAGGAGGCTTTTGTCGACGGATCCGATATCGGTACCTTGCGAACCTTCAAGTACCTT42405073.0518839567752325No Hit
TTAATATGGACTAAAGGAGGCTTTTGTCGACGGATCCGATATCGGTACCGTCGAAACGGACAACAGTTTT7250930.5218479049486355No Hit
TTAATATGGACTAAAGGAGGCTTTTGTCGACGGATCCGATATCGGTACCCTAATAATAAAAAACGTTATT7152290.5147488049236548No Hit
TTAATATGGACTAAAGGAGGCTTTTGTCGACGGATCCGATATCGGTACCGTCATAACTAGAAATAAGTTT3305590.23790261609464716No Hit
TTAATATGGACTAAAGGAGGCTTTTGTCGACGGATCCGATATCGGTACCCCCTTAACAGTCAAGCCGGTT3039850.21877736426335787No Hit
TTAATATGGACTAAAGGAGGCTTTTGTCGACGGATCCGATATCGGTACCTTCGTAAAATTCAAGTTCATT2075400.14936610089056138No Hit
TTAATATGGACTAAAGGAGGCTTTTGTCGACGGATCCGATATCGGTACCATATAAAATCGGAAAGCTTTT1824140.1312829716095734No Hit
TTAATATGGACTAAAGGAGGCTTTTGTCGACGGATCCGATATCGGTACCTGGGAAAGACAAAAAGACGTT1707920.12291864268719647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT38018950.063.5275347
CTCGATG38096200.063.4291538
GGCTCGA11981650.063.1903956
CGATGAG38255950.063.09044310
TCGATGA38329550.062.9536069
GATGAGT38311900.062.94804811
CGCTCGA7030350.062.9155656
ATGAGTT38317550.062.8956512
TAATATG19248850.062.640232
AGGATCC37983450.062.6202625
TGCTCGA11530950.062.619386
GAGTTTT38593150.062.6137914
TGAGTTT38518650.062.58392713
TCTAAGG38372900.062.57644721
GGATCCA38001150.062.54235526
AATATGG19385850.062.4747623
CTAAGGA38333950.062.4682122
AAGGATC38213750.062.44569424
AGCTCGA8125850.062.3652046
TAAGGAT38367800.062.3601523
Produced by FastQC (version 0.11.5)