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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

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        Report generated on 2018-01-23, 06:01 based on data in: /mnt/gencore/sites/core-fastqc.bio.nyu.edu/html/H3GCWBGX5/merged


        General Statistics

        Showing 27/27 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        H3GCWBGX5_n01_ncrtl01
        61.4%
        44%
        14.2
        H3GCWBGX5_n01_ncrtl02
        70.4%
        45%
        15.0
        H3GCWBGX5_n01_ncrtl03
        68.7%
        45%
        10.6
        H3GCWBGX5_n01_ncrtl04
        61.4%
        45%
        15.4
        H3GCWBGX5_n01_ncrtl05
        60.6%
        45%
        8.0
        H3GCWBGX5_n01_ncrtl06
        62.8%
        44%
        12.2
        H3GCWBGX5_n01_ncrtl07
        63.2%
        45%
        10.3
        H3GCWBGX5_n01_ncrtl08
        80.9%
        45%
        23.1
        H3GCWBGX5_n01_ncrtl09
        60.7%
        44%
        14.6
        H3GCWBGX5_n01_ncrtl10
        68.4%
        45%
        11.8
        H3GCWBGX5_n01_ncrtl11
        58.8%
        45%
        8.7
        H3GCWBGX5_n01_ncrtl12
        81.1%
        45%
        12.2
        H3GCWBGX5_n01_ncrtl13
        87.6%
        51%
        11.1
        H3GCWBGX5_n01_ncrtl14
        57.0%
        44%
        9.6
        H3GCWBGX5_n01_ncrtl15
        55.6%
        44%
        6.9
        H3GCWBGX5_n01_ncrtl16
        54.8%
        45%
        8.8
        H3GCWBGX5_n01_ncrtl17
        86.8%
        46%
        14.1
        H3GCWBGX5_n01_ncrtl18
        67.0%
        46%
        17.4
        H3GCWBGX5_n01_ncrtl19
        67.0%
        45%
        19.5
        H3GCWBGX5_n01_ncrtl20
        66.6%
        45%
        14.6
        H3GCWBGX5_n01_ncrtl21
        84.5%
        44%
        13.6
        H3GCWBGX5_n01_ncrtl22
        70.0%
        46%
        16.2
        H3GCWBGX5_n01_ncrtl23
        58.9%
        45%
        15.8
        H3GCWBGX5_n01_ncrtl24
        71.4%
        45%
        8.0
        H3GCWBGX5_n01_ncrtl25
        86.5%
        44%
        5.2
        H3GCWBGX5_n01_ncrtl26
        67.4%
        46%
        11.5
        H3GCWBGX5_n01_ncrtl27
        66.0%
        45%
        14.9

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF ReadsUndetermined % PhiX Aligned
        4.0
        364342201
        352724788
        2.6
        1.7

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.
        Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

        Showing 27/27 rows and 3/3 columns.
        LibraryTotal Read CountPortion (%)Perfect (%)
        ncrtl10
        11808654
        3.4
        99.4
        ncrtl20
        14632985
        4.3
        99.4
        ncrtl16
        8829530
        2.6
        99.2
        ncrtl14
        9578375
        2.8
        99.0
        ncrtl05
        8037927
        2.3
        99.3
        ncrtl17
        14086049
        4.1
        99.3
        ncrtl08
        23138064
        6.7
        99.3
        ncrtl03
        10573943
        3.1
        99.4
        ncrtl04
        15351622
        4.5
        99.3
        ncrtl21
        13645932
        4.0
        99.4
        ncrtl13
        11066396
        3.2
        99.3
        ncrtl26
        11548442
        3.4
        99.4
        ncrtl02
        15038585
        4.4
        98.7
        ncrtl24
        8030844
        2.3
        99.2
        ncrtl06
        12187272
        3.5
        99.4
        ncrtl11
        8674613
        2.5
        99.3
        ncrtl18
        17399330
        5.1
        99.3
        ncrtl19
        19543654
        5.7
        99.4
        ncrtl23
        15836248
        4.6
        99.3
        ncrtl09
        14601274
        4.2
        99.4
        ncrtl27
        14943945
        4.3
        99.1
        ncrtl12
        12215166
        3.6
        99.2
        ncrtl15
        6851487
        2.0
        99.3
        ncrtl07
        10276818
        3.0
        99.5
        ncrtl25
        5233838
        1.5
        99.2
        ncrtl01
        14171394
        4.1
        99.5
        ncrtl22
        16248363
        4.7
        99.4

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        loading..

        Sequence Length Distribution

        All samples have sequences of a single length (75bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        loading..

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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