FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14943945
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC167280.11193831347746529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG21800.030.7037740
GCACACG40800.029.6777511
CACACGT47550.025.31972112
ACGTCTG48600.025.19832815
ACACGTC49500.024.3920513
AGCACAC53750.022.91256710
ACTCTAG100200.022.6210945
CACGTCT55450.022.02346814
ACTACCC62050.021.795176
GCTGACT57500.021.2414821
TACCCTA65850.020.3788998
CTACCCT67050.019.9627937
CGGATCG56100.019.12757937
CTAGCAG61600.019.0407968
GAGCACA66950.018.755649
CGTCTGA66950.018.60118516
CAGTCAC62450.018.45340527
GACTCTA72000.018.3998684
TCCAGTC70100.017.71884325
TACTCTA59250.017.5264574
Produced by FastQC (version 0.11.2)