H3GCWBGX5_n01_ncrtl26.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3GCWBGX5_n01_ncrtl26.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11548442
Sequences flagged as poor quality	0
Sequence length	75
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	34715	0.30060331947807334	RNA PCR Primer, Index 29 (100% over 43bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC	23869	0.20668588888440534	No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG	13813	0.11960920789141946	No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC	12168	0.10536486220392327	No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG	11774	0.10195314658029196	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	5765	51.64679	46
CGTATGC	6005	49.869904	44
GTATGCC	6000	49.738956	45
TCGTATG	6625	44.994724	43
CTCGTAT	6590	44.291325	42
ACGTCTG	6975	44.01879	15
GCACACG	7000	43.861774	11
CACACGT	7220	42.57323	12
ACACGTC	7305	42.125076	13
CACGTCT	7595	40.65341	14
GCCGTCT	7435	39.905468	49
CGTCTGA	7790	39.3692	16
AGCACAC	7880	39.313736	10
CCAACTA	7720	39.19673	33
TCTCGTA	7685	37.891327	41
CAGTCAC	8035	37.486526	27
CAACTAA	8060	37.029404	34
ACTAATC	7985	36.29647	36
CCAGTCA	8340	36.156208	26
TCCAGTC	8470	35.76451	25