FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5233838
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1853683.5417221549463322RNA PCR Primer, Index 28 (100% over 43bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG949951.815016055139651No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT634591.2124754338976484No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT345500.660127424654718No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTG329730.6299965723050656No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACA328670.6279712899023623No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA319540.6105271122262478No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC317210.6060753122278527No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC288880.551946774049942No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAG266710.5095878015330241No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT258430.49376767106662456No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTT241320.4610765560569509No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC224680.4292834436220609No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATA222510.4251373466278475No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAG205060.39179661273428795No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG195830.3741613706805598RNA PCR Primer, Index 28 (100% over 43bp)
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGAT187320.3579017921456491No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTT184190.3519214771263459No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGT182090.34790912519646194No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT180600.34506226597002043No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCATAGGCTACTAGTGGCACACTGGGTTT165470.3161542256370946No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGG163370.31214187370721064No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154370.2949460797219937No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC149590.2858132024720674No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA139760.26703157415265816No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTA130150.24867028746399872No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTG128220.2449827449760577No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAGCAGAT127670.24393189089918335No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTGG125190.239193494334368No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAG123300.23558237759747244No Hit
GCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATC122510.23407296901432562No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT117070.22367906687215003No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAAC112420.21479457331312124No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGA106550.20357909434720753No Hit
TTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAG106290.2030823269654124No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA101930.19475192010146283No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGA101430.1937965982133952No Hit
GAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTAC94450.18046030465597138No Hit
TGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGT93730.17908464113715403No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTA90380.17268398448710107TruSeq Adapter, Index 7 (97% over 37bp)
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGT90080.17211079135426047No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGT89070.17018104114036392No Hit
CATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCA86490.16525158019793507No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCA85800.16393323599240175No Hit
GGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTC85740.16381859736583362No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTC83180.1589273492989275No Hit
AAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTA76790.14671833556942343No Hit
GCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGT73640.1406998076745975No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAGCAGATAGTACCAAACC70790.1352544729126121No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTACTAG69490.13277063600363634No Hit
CACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC66800.1276310042458326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66500.12705781111299205No Hit
GTTTGGTACTATCTGCTATGTATCCTGTCCTACTATCTAACCTCTGTAGGACTATCAGGTGTCCAGCATTAGAGG63100.12056162227413228No Hit
ATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTGGTACT62600.11960630038606468No Hit
TAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACC62080.11861276562247437No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCA62020.11849812699590626No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAG61450.11740906004350919No Hit
GATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGTATGA60670.11591875789812373No Hit
ATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCT60610.11580411927155561No Hit
CCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCT57640.11012950725643399No Hit
CTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAG57070.10904044030403692No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAGCAGATAGTACC56750.10842903429567365No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG56440.10783673472507173RNA PCR Primer, Index 28 (100% over 43bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA54370.10388170210847185No Hit
AGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTA54180.10351867979100614No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTGG53700.10260157077846123No Hit
ACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAA52960.10118769438412116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT260050.060.3267742
CGTATGC262150.060.31501444
CCGTCTT261600.060.30878450
TCGTATG262300.060.26851743
TATGCCG262850.060.18063746
TGCTTGA263350.059.96951358
GTATGCC264100.059.93441845
GCCGTCT263450.059.92457249
CTGCTTG263800.059.7762557
GCTTGAA264400.059.74440859
CTTGAAA264950.059.67360760
ATGCCGT265300.059.62487847
CGTCTTC264850.059.5285151
TGCCGTC265950.059.4650448
GTCTTCT268300.058.82565752
TCTGCTT270250.058.38731856
CTTCTGC270300.058.30105254
TTCTGCT271950.057.9594655
TCTCGTA271150.057.73050341
TCTTCTG274200.057.5850653
Produced by FastQC (version 0.11.2)