FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8030844
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG326290.4062960256730177No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT233450.29069173800412507No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT172830.21520776645642725No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC158860.19781233454416497No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA140830.17536139414487442TruSeq Adapter, Index 27 (97% over 44bp)
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT138680.172684216005192No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACA133470.1661967285132173No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC118890.14804172512876607No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA117520.14633580231417767No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC103550.12894037040191542No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTG97390.12126994373194151No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAG95080.11839353373070127No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT87180.10855646056628669No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAG81060.10093584186170221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC30700.045.17697546
TATGCCG30800.044.6939748
GTATGCC31700.043.75155647
TCGTATG32800.042.49571245
CTCGTAT33600.041.07291844
GCACACG38250.037.33904311
TATCTCG37700.036.42329841
ACGTCTG39050.036.39763615
ACACGTC40250.035.2265613
CACGTCT41600.034.6647514
GCCGTCT39600.034.5866551
CACACGT41750.034.04356412
ACTCTAG130150.033.877455
TACTCTA116950.033.3056374
TTGTACT117850.033.1131361
AGCACAC43550.032.87412610
GTACTCT118700.032.843883
TCTCGTA43000.032.4153343
GTCACAT43950.031.95075829
CAGTCAC44950.031.77724827
Produced by FastQC (version 0.11.2)