FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15836248
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC183870.11610704757844155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25950.026.05871646
ACTCTAG100450.025.4160375
GCTGACT59500.024.4136281
GCACACG39950.024.35071811
GTATGCC28050.023.49294945
CGTATGC29300.022.25539444
CTAGCAG66350.021.8370848
ACGTCTG45350.021.29931615
TACCCTA55200.020.5609468
CACACGT48500.020.342612
AGCACAC48200.020.32594510
GACTCTA76900.019.8299674
ACTACCC58500.019.5798026
ACACGTC50600.019.29399713
TACTCTA59600.018.9868814
CTGACTC80000.018.6302972
CTACCCT62350.018.2588487
CTAGTTG60150.018.1806728
CACGTCT55050.017.6716914
CTATCGA81950.017.1331517
Produced by FastQC (version 0.11.2)