FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16248363
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA877130.5398266890024552TruSeq Adapter, Index 25 (97% over 44bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC341330.21007039293743007No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG278440.17136495534965582TruSeq Adapter, Index 25 (97% over 44bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG224870.13839548020929862No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG166710.10260110510824999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC165000.058.33667846
TATGCCG164850.058.24218848
GTATGCC167150.057.50321647
GCACACG176750.054.98050711
TCGTATG176300.054.6368845
CTCGTAT175650.054.52546744
ACGTCTG182750.053.17540415
CACACGT183150.053.1536112
ACACGTC184500.052.67119213
TATCTCG182100.052.5582241
GTCACAC187900.051.56240529
GCCGTCT188100.051.1524551
CACGTCT189900.051.11924714
AGCACAC192050.050.8159410
TCTCGTA189300.050.57568743
ACTGATA194750.049.6783834
CAGTCAC196850.049.25266627
CGTCTGA198750.048.96404316
CACACTG200750.048.24604831
CCAGTCA201950.047.97379326
Produced by FastQC (version 0.11.2)