FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13645932
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG6829845.00503739869142RNA PCR Primer, Index 24 (100% over 43bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG1378221.0099859797044277No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT1269810.9305410579504573No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA669080.490314622702209No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC650100.47640571563745154No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT649680.4760979316033526No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC582460.4268378297649439No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTG579520.4246833415262512No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT516040.3781639832295808No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATA485140.3555198721494435No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC482100.35329210199787014No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACA473560.3470338266378581No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440450.32277018528305723No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAG435720.31930395080380003No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTT418440.30664083625801447No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT375690.27531281850151385No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTT363910.266680209164167No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG358450.2626790167208806RNA PCR Primer, Index 24 (100% over 43bp)
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAG327290.23984437266725353No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGG315850.2314609218337011No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC312980.2293577309340249No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGAT295080.21624026852837902No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA271390.19887978336694043No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCATAGGCTACTAGTGGCACACTGGGTTT260820.1911338851754501No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA246420.18058128972062884No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT238340.17466011115986801No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGGGTTTGG233210.17090074902908792No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAGCAGAT213980.1568086371821287No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTA206840.1515763086024465No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTG196230.1438010976458039No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCA188670.13826098503202272No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAG183270.13430376173646474No Hit
GCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATC182040.1334023942080321No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA177290.1299215033461987No Hit
TTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAG169750.12439604711499369No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGA165110.12099576635732905No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGA163020.11946417437812236No Hit
AAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTA162940.11940554884781779No Hit
TGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGT160970.11796189516406794No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGT160850.1178739568686111No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAAC158050.11582206330795142No Hit
GAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTAC156150.11442970696321805No Hit
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGT153170.11224590595937309No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG149080.10924867572255233RNA PCR Primer, Index 24 (100% over 43bp)
CATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCA139330.10210368921668377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT907050.063.8230942
TCGTATG913600.063.78069343
CGTATGC915700.063.72674644
TATGCCG915400.063.6755646
TCTCGTA908050.063.62501541
CCGTCTT906000.063.52588350
GCCGTCT905000.063.4936249
ATGCCGT916450.063.3861647
GTATGCC922400.063.35713645
ATCTCGT914350.063.29252640
CGTCTTC903050.063.20551351
TGCCGTC920500.062.8442448
CATCTCG921300.062.82301339
ACGGTAG948900.062.58358832
CACGGTA950100.062.48638531
CGGTAGC949450.062.42672333
TAGCATC935000.062.3722936
AGTCACG955500.062.1747628
GTAGCAT949450.062.0887535
ACGTCTG963300.062.0691715
Produced by FastQC (version 0.11.2)