FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14632985
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1648231.1263798876305824TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG513890.35118603620518984TruSeq Adapter, Index 7 (97% over 36bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC204090.13947256831056684No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT178640.12208035476015318TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG293650.061.3397248
CGTATGC298050.060.53940646
GTATGCC297650.060.5162347
CTCGTAT303600.059.2401344
TCGTATG307100.058.8454345
GCACACG311450.058.70472311
AGTCACG311450.058.33709728
ACGTCTG314550.058.1269615
ACACGTC317000.057.7097713
TATCTCG311350.057.61139741
ACGAGTG315350.057.53943632
CGAGTGG316600.057.2684733
CACGAGT318200.057.15419431
CACACGT320500.057.13337312
GCCGTCT317650.056.60707551
CACGTCT323450.056.5275514
AGCACAC324800.056.34493310
CGTCTGA327200.055.91151416
TCTCGTA321750.055.90986343
CAGTCAC329850.055.1667527
Produced by FastQC (version 0.11.2)