FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19543654
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA826690.422996641262683TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG252650.1292746996032574TruSeq Adapter, Index 22 (97% over 40bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC218370.1117344791306682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG173300.053.7708248
GTATGCC175850.053.07078647
CGTATGC177100.052.5407646
GTACGTA183200.051.0583735
CGTACGT185050.050.43578734
TCGTATG190650.048.98751445
CTCGTAT190550.048.63361444
TACGTAA193400.048.36552436
CCGTACG194550.048.09724433
GCACACG209950.047.55139511
ACGTCTG215650.046.23054515
ACACGTC218350.045.6591213
CACACGT219950.045.40527712
ACCGTAC209600.044.77561632
AGCACAC224600.044.6340710
GCCGTCT208100.044.5627851
TCTCGTA209450.044.2621443
CACGTCT226600.043.9814314
CGTAATC216300.042.8935138
CGTCTGA234250.042.66292616
Produced by FastQC (version 0.11.2)