FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17399330
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC370220.2127783081302556No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA241990.13908006802560788TruSeq Adapter, Index 20 (97% over 44bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG193470.11119393677802537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG63650.045.36752348
CGTATGC66550.043.33960346
GTATGCC68250.042.41136647
GGCCTTA71000.040.7713736
GCACACG73700.040.44273811
GTCACGT76250.038.59753829
CGTGGCC76800.037.73753433
CTCGTAT77200.037.45078744
TCGTATG78050.037.3519345
ACGTCTG80350.037.009515
GTGGCCT80250.036.24384334
AGTCACG82050.036.079828
CACACGT82800.035.9981512
ACACGTC83950.035.5872113
TCACGTG82300.035.55076630
GCCTTAT82100.035.09124437
TATCTCG82700.034.9192941
AGCACAC86400.034.61781310
CACGTCT88700.033.6815714
CACGTGG87000.033.59054631