Basic Statistics
Measure | Value |
---|---|
Filename | H3GCWBGX5_n01_ncrtl18.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 17399330 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC | 37022 | 0.2127783081302556 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 24199 | 0.13908006802560788 | TruSeq Adapter, Index 20 (97% over 44bp) |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG | 19347 | 0.11119393677802537 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 6365 | 0.0 | 45.367523 | 48 |
CGTATGC | 6655 | 0.0 | 43.339603 | 46 |
GTATGCC | 6825 | 0.0 | 42.411366 | 47 |
GGCCTTA | 7100 | 0.0 | 40.77137 | 36 |
GCACACG | 7370 | 0.0 | 40.442738 | 11 |
GTCACGT | 7625 | 0.0 | 38.597538 | 29 |
CGTGGCC | 7680 | 0.0 | 37.737534 | 33 |
CTCGTAT | 7720 | 0.0 | 37.450787 | 44 |
TCGTATG | 7805 | 0.0 | 37.35193 | 45 |
ACGTCTG | 8035 | 0.0 | 37.0095 | 15 |
GTGGCCT | 8025 | 0.0 | 36.243843 | 34 |
AGTCACG | 8205 | 0.0 | 36.0798 | 28 |
CACACGT | 8280 | 0.0 | 35.99815 | 12 |
ACACGTC | 8395 | 0.0 | 35.58721 | 13 |
TCACGTG | 8230 | 0.0 | 35.550766 | 30 |
GCCTTAT | 8210 | 0.0 | 35.091244 | 37 |
TATCTCG | 8270 | 0.0 | 34.91929 | 41 |
AGCACAC | 8640 | 0.0 | 34.617813 | 10 |
CACGTCT | 8870 | 0.0 | 33.68157 | 14 |
CACGTGG | 8700 | 0.0 | 33.590546 | 31 |