FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8829530
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA117430.1329968865839971TruSeq Adapter, Index 18 (97% over 40bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC102890.11652941889319136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC30450.049.40614733
GTCCGCA32600.046.14750734
CGCACAT33350.045.72996537
TATGCCG33800.044.70722648
ACGTCCG34000.044.55227332
TCCGCAC34550.043.7424335
CCGCACA34900.043.4031236
CGTATGC35850.042.1519446
GTATGCC36650.041.32527547
GCACACG38800.040.7211111
ACGTCTG41900.037.62536215
CACGTCC41100.036.93943431
AGTCACG42300.036.4612628
CACACGT44600.036.04412512
TCGTATG42050.035.8550745
AGCACAC44800.035.65240510
CTCGTAT42550.035.5154244
ACACGTC45150.035.14681213
GTCACGT44500.034.504229
TCACGTC45850.033.41357830
Produced by FastQC (version 0.11.2)