FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6851487
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112170.1637162852385183No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC92590.13513854729637523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG47850.028.1194365
GCTGACT29400.026.5220581
CTAGCAG30150.024.4860488
GACTCTA35100.022.1156314
CTAGTTG28450.020.9776158
ACTACCC26350.020.1634146
TAGCATG35600.020.15826627
CTATCGA36400.019.90276117
TACTCTA29500.019.8815784
TACCCTA26450.019.8248628
TATGCCG10800.019.48647146
CTACCCT26950.019.329337
TCTATCG39450.018.53877816
GGACACC29000.018.44134129
CTGACTC41350.018.4391422
CTCTAGT34000.018.3663836
CTAGCAT39800.018.20425426
GCATGAC40300.017.97931329
GACACCT31000.017.69690930
TCTAGTT36000.017.6325027
Produced by FastQC (version 0.11.2)