FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9578375
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG55950.026.082695
CTAGTTG34750.023.3294648
GCTGACT33450.020.2174551
TACTCTA39550.020.1501184
GGACACC39500.019.04238729
TACCCTA23250.018.1020838
TCTAGTT47500.018.0841227
ACCTCTA42350.018.08386417
GACTCTA41250.017.8142594
CTAGCAG37000.017.71518
GACACCT42550.017.59642430
CTCTAGT49600.017.458396
GTTACCT44250.017.2297814
GTACTCT47200.017.0304473
CTAATGC46250.016.55964721
TTGTACT49550.015.9462421
ACTACCC28300.015.7258956
CTATCGA42200.015.69569217
TAGTTGT54150.015.5447269
TGTACTC51300.015.5348372
Produced by FastQC (version 0.11.2)