FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11066396
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC202440.18293218496789743No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT174150.15736830671882696No Hit
GCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCGTATCGCCGACCGAAGGGACAAGCCGACCAATGCACACC143140.1293465370297611No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCGTTAGGCGCGTGCTGCAGACCACGAT137330.12409640862300608No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC134760.12177406266683388No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC131190.11854808015183986No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCTTCGAT127840.11552089767978664No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT127460.11517751578743432No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGA126530.11433713378773No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCGT117560.1062315138550979No Hit
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCCCGAGAGAGCCGTTCCCAGTCCGTCC117460.10614115019921572No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111670.10090909452363714No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG111430.10069222174951989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT56950.036.831713
TACTCTA60950.034.9804044
TCTAGTT63450.033.328627
TATGCCG7300.033.0822548
TAGTTGT66050.031.6503459
GTTACCT65600.031.23726514
CTAATGC66050.031.13101621
TACCTCT66650.030.90001716
CGTATGC8150.030.0562246
TGTTACC68450.029.8858613
ATGCTGG69150.029.68739724
TGGACAC67150.029.59590728
TAATGCT69850.029.43861622
GTATGCC8250.029.27317447
ACCTACT19300.029.14038533
TCTAATG70500.029.11720520
AATGCTG74550.027.67546823
GTTACTA60700.027.4517773
CTGGACA75250.027.05232827
ACTACCC62400.026.9802076
Produced by FastQC (version 0.11.2)