FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12215166
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT196680.16101295717143754No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG175290.1435019384918715No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC147310.12059598698863364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA96900.034.4296044
ACTCTAG130250.033.904835
GTACTCT103600.032.269723
TTGTACT104500.031.4343971
CTAGTTG108100.030.9557698
TCTAGTT110050.030.8147777
TGTACTC109550.030.2967192
CTCTAGT113950.029.4293926
ACTACCC101350.028.4925336
TAGTTGT118800.028.4575869
GTTACCT115400.028.39967314
CTAATGC116150.027.86170421
CTGTTAC105850.027.7084671
ACCTCTA118500.027.59838917
TACCTCT120400.027.21984116
GGACACC114350.027.18595529
TGTTACC123050.026.60592513
CTACCCT109450.026.602497
ATGCTGG123150.026.0545324
TGGACAC121150.025.91625228
Produced by FastQC (version 0.11.2)