FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8674613
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCC32050.022.819986
TACCCTA32400.022.6791638
TACTCTA38800.022.31774
CTAGTTG39550.021.8937248
GGACACC38500.021.23929629
ACTCTAG44600.020.807745
TCTAGTT44550.020.2883177
CTAATGC41900.020.00781421
CTACCCT36600.019.7938677
ACCTCTA43550.019.40753417
GTACTCT45050.019.374733
CCTACAC40650.019.34925311
GACACCT43400.018.9209230
TGGACAC45150.018.41669828
GCTGGAC44500.018.29803726
GTTACCT47000.018.20324314
CTCTAGT49700.018.1866366
TTGTACT48200.017.5376551
TACCTCT49250.017.5113116
ATGCTGG48650.017.44525124
Produced by FastQC (version 0.11.2)