FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11808654
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC122770.10396612518242976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG79850.026.6575155
TACTCTA53650.022.4421844
TACCCTA53900.022.0165718
ACTACCC54900.021.9940456
GCTGACT42050.021.9897631
CTAGTTG53250.021.896628
CTACCCT56850.021.1778727
GGACACC54100.020.15359729
CTAGCAG47050.019.7229848
GTACTCT60750.019.4786593
TCTAGTT64750.019.3931777
CCTACAC64800.019.05830611
CTGTTAC65650.019.0250381
GACACCT58800.018.71879830
CTAATGC61950.018.65583821
GACTCTA54750.018.3365784
TACTACC65100.018.3359975
CTCTAGT69000.018.2995936
TTGTACT63900.018.0882131
TGTACTC64550.018.0647412
Produced by FastQC (version 0.11.2)