FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14601274
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG78500.020.8764155
TACCCTA44150.018.2057918
ACTACCC46550.017.7137366
GCTGACT51400.016.98331
CTAGCAG53950.016.1136328
CTACCCT50800.015.8228787
GACTCTA59350.015.7536264
CCTACAC58850.015.00644611
CTAGTTG52800.014.7658638
TACTCTA56400.014.4365974
CTATCGA64250.013.36967917
TAGCATG61700.013.253127
GCGGGCC5550.013.0554241
GGACACC53500.012.83379429
CTAGCAT63000.012.81519126
TACTACC63800.012.7621335
TCTATCG67150.012.68931716
CTGACTC69100.012.5823112
GTGCGCA38350.012.41407668
CTAATGC60300.012.35802521
Produced by FastQC (version 0.11.2)