FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23138064
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC349520.15105844637649893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG187850.031.056615
GCTGACT97300.026.7731151
TACTCTA120550.026.3867194
CTAGTTG124800.025.1822998
GACTCTA117150.023.5891274
CTAGCAG111650.023.5753128
TCTAGTT139500.023.295257
GTACTCT139300.022.7855383
CTCTAGT144550.022.6262156
TTGTACT139800.022.4347671
TGTACTC140900.022.4288522
ACCTCTA140000.021.80759417
GGACACC132450.021.54323429
CTGACTC127600.020.8732092
GTTACCT148150.020.74781414
CTAATGC148200.020.50878521
GACACCT142300.020.24598530
TACCCTA121750.019.6644658
TACCTCT156850.019.64068816
TAGTTGT166400.019.3634769
Produced by FastQC (version 0.11.2)