FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10276818
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC140070.13629705225878283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG67100.027.5593855
GCTGACT42100.025.2446781
TACTCTA37100.022.7834474
CTAGCAG46550.022.084418
GACTCTA50700.021.9116544
CTAGTTG40700.020.342588
CTGACTC55850.018.7176322
ACTACCC32300.018.585496
GGACACC41450.018.14597729
TACCCTA32700.017.9345478
TCTAGTT50050.017.714247
ACCTCTA46700.017.5814917
CTATCGA59700.017.509117
CTACCCT34250.017.425257
GTACTCT48200.017.3219873
CTAATGC46250.017.08231721
TGTACTC49900.016.66282
TAGCATG61600.016.63513227
GTTACCT49000.016.61542714
CTCTAGT51800.016.584296
Produced by FastQC (version 0.11.2)