FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12187272
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA29450.016.6335358
ACTACCC31800.016.382276
CTAGTTG40900.015.6037858
TACTCTA42900.015.2799024
GGACACC39400.015.23716229
GCGGGCC4051.4551915E-1114.4832581
ACTCTAG48700.014.0977055
GACACCT43050.013.78521230
CTACCCT36300.013.7802527
CCTACAC40050.013.52317411
ACCTCTA47300.013.34700917
TCTAGTT53000.013.2785337
CTAATGC48750.013.16328921
GTACTCT52200.012.49152853
GTTACCT51100.012.4217814
GCTGGAC51200.012.19703626
CTCTAGT54800.012.1506936
GCGGGAT23900.011.8382731
ATGCTGG52300.011.61051124
CGCGGGG8400.011.5014111
Produced by FastQC (version 0.11.2)