FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8037927
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC88460.1100532512922797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG58450.029.8059335
TACTCTA41050.026.3883574
CTAGTTG42600.025.1840218
TCTAGTT49150.023.0214527
GTACTCT46950.022.9252913
TGTACTC47950.022.4471862
GCTGACT30450.022.4346711
CTCTAGT49550.022.418586
TTGTACT49200.021.8791941
GGACACC46150.021.3832629
ACTACCC40900.021.171246
CTAATGC49300.020.99305321
CTAGCAG33100.020.7395528
GTTACCT51400.020.537314
TACCCTA41250.020.4887948
ACCTCTA51700.020.41774717
ATGCTGG52650.019.92139224
GACACCT49050.019.90822230
CTACCCT43150.019.826837
TACCTCT54000.019.61210816
Produced by FastQC (version 0.11.2)