FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15351622
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG75500.021.8882355
GCTGACT53300.020.585381
CTAGCAG60800.017.475798
GACTCTA68200.017.4018864
TAGCATG66100.015.60692727
CTATCGA67700.015.5927417
GTGCGCA46150.015.17478368
TCTATCG70600.015.09858916
CTGACTC74750.014.8616452
TACCCTA28300.014.7498858
GGTGCGC47200.014.47171467
CTAGCAT72150.014.29809926
CTAGTTG42650.014.1549638
ACTACCC31250.013.8000986
TGCGCAT52950.013.22594869
GCATGAC79650.013.03852429
GCCGGGG16850.012.4907631
CTACCCT35150.012.4643047
CTCTAGC93200.012.3268066
TACTCTA49050.012.3089564
Produced by FastQC (version 0.11.2)