FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10573943
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC160890.1521570524826926No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT119350.11287180193802822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG76500.028.771375
GCTGACT46150.026.7649211
TACTCTA41600.023.5518074
CTAGTTG41600.023.2194338
GACTCTA55650.022.7509824
CTAGCAG53500.022.2460168
CTGACTC58800.020.8284262
TACCCTA36600.020.0763828
ACTACCC37150.019.7798086
GGACACC44250.019.64897529
ACCTCTA47350.019.52566517
GTACTCT50700.018.9845183
TCTAGTT53300.018.9637977
CTAATGC47850.018.96259321
GACACCT47350.018.72698430
CTCTAGT53850.018.514566
CTATCGA66350.017.7818217
CTCTAGC70350.017.6048226
ATGCTGG51550.017.60205324
CCTACAC47150.017.55992311
Produced by FastQC (version 0.11.2)