FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15038585
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC162550.10808862668927961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG113200.025.9974445
GCTGACT57350.023.0422691
TACTCTA77800.021.5962054
CTAGCAG63150.020.7584538
CTAGTTG79850.020.0028028
TACCCTA70350.019.9088738
ACTACCC71400.019.7630146
GACTCTA69500.019.211194
GTACTCT88950.018.966733
CTACCCT75700.018.5023337
TCTAGTT90550.018.4015757
TTGTACT87150.018.3700541
CTGACTC75700.017.728962
TGTACTC94050.017.4980282
GGACACC84100.017.476829
CTAATGC90800.017.43981721
GTTACCT92250.017.23957314
CTCTAGT97650.017.1708626
ACCTCTA93300.017.04561617
GACACCT88000.016.97676730
Produced by FastQC (version 0.11.2)