FastQCFastQC Report
Tue 23 Jan 2018
H3GCWBGX5_n01_ncrtl01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3GCWBGX5_n01_ncrtl01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14171394
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG48700.018.9131688
TACTCTA50800.018.4039294
TACCCTA40750.017.1850368
ACTACCC42050.016.654636
TCTAGTT62650.016.4639917
ACTCTAG62000.016.414795
GGACACC50600.016.2971129
GACACCT53900.015.49147930
CTCTAGT65850.014.8787876
GTACTCT65450.014.8116413
CTAATGC60050.014.53532421
CCTACAC55800.014.3428111
ACCTCTA62550.014.28445217
GTTACCT61850.014.27848914
CTACCCT49900.014.1031037
GCTGGAC60550.013.67608626
TGTACTC69150.013.4703252
ATGCTGG64450.013.43669824
TGTTACC68950.013.15836513
TAGTTGT73400.013.0655959
Produced by FastQC (version 0.11.2)