FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic898.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic898.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26184621
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCAG247300.012.54277269
ATATGCA353450.011.77273469
GATATGC380950.011.57499769
ATGCAGT221550.011.41537569
TGCAGTG169150.011.13726669
GCAGTGT139000.010.94666469
CGATATG462650.010.70181269
GTATTAG55800.010.6458351
GTATAGA72700.010.5463961
GTATAAG53400.010.4775381
CAGTGTG131300.010.40611169
ACGATAT552900.010.31535669
GTATATA96150.010.0216771
GTATTAT111300.09.9297951
GTATAGG38550.09.8549361
GTATAAA129450.09.7648271
CTGCCGC64950.09.61517969
TTATACT126900.09.3788574
GACGATA622500.09.13431268
GTATTAC65950.08.9550261
Produced by FastQC (version 0.11.2)