FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic773.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic773.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27930701
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGTG277250.013.29079369
TATGCAG408500.012.9226269
ATATGCA561450.012.91118769
GCAGTGT220200.012.660336569
ATGCAGT355550.012.35318469
GATATGC647900.011.94994969
CAGTGTG195550.011.90959969
CTGCCGC78550.011.46424669
GTATTAG60500.011.0183011
GTATTAT112650.010.9765171
CGATATG787450.010.92759769
GTATAAG55000.010.9269581
TTATACT132550.010.6705144
ACGATAT921200.010.61818669
GTATATA97350.010.111641
GTATAAA128300.09.9875731
TACACAG80650.09.7089525
CGCCGTA22800.09.533565567
ACAGTGC65600.09.412568
GTATAGG49200.09.4070271
Produced by FastQC (version 0.11.2)