FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33742973
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCAG244350.011.21117169
GATATGC374050.011.00407769
GTATATA131700.010.5420641
GTATAAG70450.010.4420061
TGCAGTG177950.010.4116469
TTATACT162000.010.3708794
GCAGTGT144300.010.20949869
CGATATG445450.010.06127569
GTATTAT147550.010.0182061
ATATGCA354400.09.81317769
CTTACGC59250.09.78201545
GTATATG102800.09.7765421
GTATTAG79800.09.6946131
ATACCCC101750.09.6630024
ACCCCAC97500.09.4109396
GTATAGA93600.09.4091351
TACCCCA104600.09.398915
ACGATAT520850.09.32017969
GTATAAA169400.09.3172591
ATGCAGT231000.09.29011769
Produced by FastQC (version 0.11.2)