FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41670945
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCAG326550.011.263062569
GATATGC495300.011.18035769
ATATGCA463550.010.6510469
GTATATA161000.010.42622951
GTATAAG81900.010.4166951
GTATTAT175700.010.399221
CGATATG595250.010.26521769
ACGATAT701600.09.92877669
ATGCAGT306900.09.90439669
GTATAAA194700.09.7924191
TGCAGTG247150.09.78602669
GTATTAG92650.09.72997951
GTATAGA112950.09.7242871
GCAGTGT195750.09.7117869
TTATACT199600.09.644114
GTATTAC97500.09.4585281
GTATAAT215150.09.4395851
GTATTAA110500.09.4085161
GTATAGG63100.09.3054291
TAATACT147850.09.2864614
Produced by FastQC (version 0.11.2)