FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic587.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic587.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30214608
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA119950.011.6903641
GTATTAG69350.011.4048941
GATATGC337800.011.34767969
TATGCAG222600.011.25289169
TGCAGTG156100.011.09566869
GTATTAT132800.010.5331731
CGATATG411950.010.3436869
CTTACGC53850.010.12213845
TTATACT145900.010.1199544
ATATGCA323550.09.981369
ATGCAGT206750.09.94611369
ACGATAT486150.09.92887569
GTATAAT159850.09.8310761
GTATAGA85850.09.7761581
CTGAGGC49200.09.74770469
GCAGTGT125650.09.69314969
GTATTAC78000.09.6087611
GTATAAA151150.09.5971751
AGGCTGA95850.09.431025566
CCTATAC48350.09.4195493
Produced by FastQC (version 0.11.2)