FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic568.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic568.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29338412
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCAG334900.013.50673569
TGCAGTG226300.012.27366369
GCAGTGT181350.012.19560369
GATATGC519750.011.99572469
ATATGCA463700.011.85336169
ATGCAGT292400.011.52869269
CGATATG622250.010.70730669
CTGCCGC75650.010.67258869
GTATATA114650.010.663131
GTATTAT127000.010.6323321
CAGTGTG167050.010.61645469
GTATAAG58800.010.3369131
ACGATAT741850.010.27871969
GTATTAA80500.010.2531551
TTATACT140800.010.0697084
GTATAAA140900.09.9755971
GTATTAG64900.09.9506721
GTATATG81650.09.8126731
GTATTAC69500.09.7392781
CTTACGC54950.09.60587645
Produced by FastQC (version 0.11.2)