FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28777632
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCAG244300.012.62583969
GATATGC384200.012.08742769
ATATGCA352500.011.74541469
TGCAGTG168150.011.73668369
ATGCAGT222800.011.47490969
CGATATG470900.010.90968569
GTATATA107250.010.8843231
GTATTAG64450.010.8245651
GCAGTGT133400.010.68170269
ACGATAT559250.010.64214869
GTATTAA76950.010.3228881
CTGCCGC72200.010.17861469
GTATAGA83300.010.0749591
GTATTAT121600.09.8270811
GACGATA632700.09.39044268
GTATAAA135300.09.2659681
TTATACT138350.09.1762134
ACCGTGC25650.09.1449738
CAGTGTG131050.09.135632569
GTATAAG58350.09.1151091
Produced by FastQC (version 0.11.2)