FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic530.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic530.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35083448
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCAG291750.012.4056569
GATATGC458900.011.64630869
TGCAGTG204400.011.12396169
GTATATA135350.011.100771
GTATAAG81900.011.049421
CGATATG548950.010.93003969
TTATACT185000.010.8712924
ATATGCA423100.010.80507569
GTATTAG75800.010.3893351
ATGCAGT268300.010.3264269
TATACTG130900.010.1981275
GCAGTGT170050.010.06375369
GTATTAT159550.09.8933071
ACGATAT651500.09.72328969
GTATTAA97800.09.2883591
CAGTGTG164350.09.04820669
ATAATAC158800.09.0380523
GTATATG98600.09.0028141
TATACAG107850.08.9874245
TAATACT131300.08.8804784
Produced by FastQC (version 0.11.2)