FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13600513
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138810.10206232661959147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCAG209150.013.41124869
ATGCAGT179050.013.08374369
ATATGCA283000.013.06907769
GCAGTGT110800.012.45537569
CAGTGTG96200.012.44488769
TGCAGTG138150.012.41200769
CTGCCGC43700.012.39523569
GATATGC328000.011.88614869
GTATATA50500.011.2854731
CGATATG395900.011.12863669
GTATTAT52950.010.632831
TTATACT64100.010.6022984
GTATAAG27850.010.541951
GTATAAA58900.010.4383531
GTATAAT64250.010.4293161
ACGATAT466700.010.039186569
TATAATA70950.09.7769472
GTATTAC32250.09.6391791
TAATACT44800.09.6255124
ATAATAC58250.09.5955183
Produced by FastQC (version 0.11.2)