FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23303355
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCAG184450.011.67226569
GTATTAG50900.011.5355761
GATATGC283000.011.399199569
ATATGCA264000.011.04337969
ATGCAGT170450.010.78895669
TGCAGTG128600.010.54388869
GTATAAA111600.010.151211
CGATATG339700.010.07547169
GTATAGA64250.09.94504451
TACCCCA69100.09.5350815
CTATACT51450.09.5215454
GTATATA88950.09.5132361
ACGATAT401400.09.39489769
ATACCCC65700.09.3467184
GTATATG67250.09.2959641
GCAGTGT105100.09.29036869
TATACAG70100.09.202225
ACCCCAC63250.09.1625416
TCTATAC62950.09.1531953
TTATACT116250.09.1403384
Produced by FastQC (version 0.11.2)