FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_nic1112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_nic1112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30837130
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG522620.1694775097423139No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA44650.014.45014667
GTATAAG80400.012.5434191
GTATAGA91600.012.1408611
TTATACT169950.012.0167724
GTATTAG66400.011.4430991
GTGTATA86700.011.3132751
GTATATA128400.011.1896731
ATACTCC431150.011.1472569
GTATTAT133650.011.1377481
GCCGTAT55950.011.0383968
TAATACT112450.010.9827434
CTATACT72500.010.848864
GTATTAA83750.010.7632841
TATACTG117700.010.697285
GTATAAT173500.010.5702231
ATAATAC142050.010.5413363
GTATAAA161500.010.4574411
GATACTC526850.010.44524869
CGATACT569200.010.35304369
GTATATG87650.010.1267551
Produced by FastQC (version 0.11.2)