FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n02_ju1872.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n02_ju1872.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26321745
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323800.12301616021278225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA104650.012.7380591
GTATAGA77550.011.7119471
GTATTAT117900.011.3358051
GTATATG77350.011.2064641
TTATACT142300.011.1275984
GTATAAG62500.010.9405811
TATACTG95800.010.5861775
TAATACT97700.010.5577234
GTATTAA68150.010.5402931
GTATTAG59850.010.4440671
GCTTAAG76900.010.31926568
GTATAAA134150.010.22009851
CTTAGGT70400.010.14479569
TACCCCA94600.010.1370355
ACCCCAC83850.010.1201876
TCTATAC75450.010.1060813
ATACCCC91850.010.0658234
GTATAGG42350.010.0301381
CTAATAC63200.09.772053
TATTATA161100.09.7685212
Produced by FastQC (version 0.11.2)