FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic898.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic898.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26184621
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGCA293950.013.63633969
CAGGCAG232500.013.18997569
GACAGGC343600.012.73008469
AGGCAGA213700.011.65461369
GTATTAG56900.011.58336451
GTATATA97300.011.2778911
AGACAGG453900.010.95138369
GTATTAT107850.010.7825981
GTATTAA71100.010.1920921
TTATACT128350.09.9994884
GAGACAG602350.09.67838769
GGCAGAA205200.09.46446869
GTATAAT130600.09.459181
GTATAAA130650.09.2970881
CGAGACA626800.09.2403768
GTATAGA73450.09.2082671
GTATTAC61900.09.1425391
GTATTGG80800.09.0966591
GTATATG76600.09.0097931
GTATAAG56650.08.95428851
Produced by FastQC (version 0.11.2)