FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic773.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic773.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27930701
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGA518600.013.84888569
CGTAGAG409550.013.00489869
GACGTAG612800.012.76142969
GTAGAGG359550.012.23256369
GTATTAT112750.011.7216081
AGACGTA744900.011.452369
TAGAGGA330150.011.26351769
GTATATA100300.010.9059451
GTATAAA126350.010.842271
GAGACGT928450.010.54436569
GTATAAT132600.010.1490651
GTATTAA70550.010.07571
TTATACT131400.010.0298144
GTATTAG61500.09.9873411
TAATACT102000.09.9780744
ACAGTGC67450.09.9217778
GTATAGT64050.09.9129671
GTATAGA71750.09.7148121
GTATAGG47900.09.6532661
TACACAG80400.09.6120675
Produced by FastQC (version 0.11.2)