FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33742973
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA131100.011.4499011
GGCATAG142050.010.90580855
GTATAAG72000.010.7357071
AGGCATA160650.010.502213554
GTATTAG73250.010.4111771
TAGGCAT174850.010.39936253
GCATAGG129450.010.34219450
GTATTAT147300.010.3077791
TTAGGCA118250.010.09606546
TTATACT172000.010.0693854
GCCATAG55200.010.0614367
GTATAAA162700.010.0320281
AGACCTC439550.09.90424669
CATAGGC127400.09.88569251
TACCCCA110350.09.72333055
GTATAGA87950.09.6519361
ACCCCAC102700.09.5741476
CACGAGC77950.09.55890269
GAGACCT492800.09.52703669
ACCTCTC350650.09.47376869
Produced by FastQC (version 0.11.2)