FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41670945
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC341850.014.03659169
CGCTACG272050.013.09850469
GCTACGC224800.013.028110569
CTACGCT180550.012.78199769
GACGCTA430000.012.73949269
TACGCTA142050.012.4579469
ACGCTAT131850.011.642669
GTATTAT183000.010.8425961
GTATATA160350.010.7601321
AGACGCT569600.010.66496669
TTATACT195100.010.6456034
GTATTAG87250.010.362211
GTATAAA197200.09.8343561
TATACAG129350.09.7354135
GAGACGC735900.09.64240969
TAATACT146700.09.6188754
GTATTAA107200.09.5926371
TATTATA234350.09.4811342
GTATAAT211750.09.3215711
ATAATAC176750.09.1156583
Produced by FastQC (version 0.11.2)