FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic587.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic587.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30214608
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGGC188800.013.3745369
CGAGGCT154350.013.09665669
ACCGAGG237600.013.0376569
GTATATA115800.012.7244071
GACCGAG304400.012.30706769
GGCATAG144000.012.12413955
GCATAGG135100.011.95235256
TAGGCAT169700.011.93528553
AGGCATA164200.011.70430554
GTATTAG67150.011.4084111
GTATTAT132850.011.2731781
GTATAAA146350.011.0113951
TTAGGCA117600.010.85595546
CATAGGC125900.010.77084451
GTATTAA76600.010.7217661
AGACCGA382950.010.52131969
GAGACCG453100.010.44550569
GTATAAG64450.010.3871651
TTATACT149300.010.3061784
TAAGCCA125700.010.18385746
Produced by FastQC (version 0.11.2)