FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic568.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic568.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29338412
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG66050.012.3818511
AGAGGCA251950.011.14494569
GTATTAT123250.011.1151061
GTATAGA80500.010.93086051
GCCGTAG20700.010.83209367
GAGGCAA204750.010.81630769
GTATAAA140150.010.5626821
AAGAGGC323150.010.55747369
GTATATA109900.010.5499791
CAAGAGG433150.010.48058369
GACAAGA627150.09.97777669
AGGCAAT161800.09.93516969
GCATAGG88200.09.89777550
ACCCCAC88700.09.879776
TTATACT147050.09.7604074
AGACAAG685800.09.73813369
TAATACT104650.09.7257364
TCTATAC77050.09.6721163
TACCCCA96250.09.6424535
GCCTTAG36950.09.61594967
Produced by FastQC (version 0.11.2)